Kevin Copeland

  • (B) HEK293T cells were transfected with SIV pDNAs expressing p27CE1 (lane 1), p27CE2 (lane 2), p57Gag (lane 3), Gag-Pro (lane 4). Lane 5 contains a sample from mocktransfected cells. Proteins from the cell-associated (prime panel: 1/100 from the sample) and extracellular (bottom panel: 1/250 from the sample) have been analyzed. Western immunoblots…[Read more]

  • Lane 5 contains a sample from mocktransfected cells. Proteins in the cell-associated (top panel: 1/100 in the sample) and extracellular (bottom panel: 1/250 on the sample) were analyzed. Western immunoblots had been probed making use of the mouse anti-p27Gag Ab KK64, which recognizes an epitope among aa 15180 and overlapping CE1 and CE2. Equal…[Read more]

  • 4D, 4E). The CE-specific cytotoxic T cell responses (granzyme B+) were compared with these induced by the subgroup of gag pDNA vaccinated animals, which showed constructive CE responses (Fig. 2A). We discovered a substantial raise (p = 0.018) inside the frequency of CE-specific granzyme B+ T cells induced by the p27CE pDNA vaccine (Fig. 4F). We…[Read more]

  • The Octenidine (dihydrochloride)MedChemExpress Octenidine (dihydrochloride) CE-specific cytotoxic T cell responses (granzyme B+) have been compared with those induced by the subgroup of gag pDNA vaccinated animals, which showed optimistic CE responses (Fig. 4F). We further noted that the gag pDNA vaccine induced a wider selection of cytotoxic…[Read more]

  • 4D, 4E). The CE-specific cytotoxic T cell responses (granzyme B+) have been compared with these induced by the subgroup of gag pDNA vaccinated animals, which showed optimistic CE responses (Fig. 2A). We found a considerable increase (p = 0.018) in the frequency of CE-specific granzyme B+ T cells induced by the p27CE pDNA vaccine (Fig. 4F). We…[Read more]

  • The DNA vaccine in macaques L986, R108 by way of R684 was administered working with the Elgen 1000 electroporation device, and in macaques T129 by means of T152 applying the CELLECTRA 5P device. No distinction within the level or nature of your induced Cetrorelix (Acetate) web CE-specific T cell responses was found working with these…[Read more]

  • Lane five contains a sample from mocktransfected cells. Proteins from the cell-associated (major panel: 1/100 from the sample) and extracellular (bottom panel: 1/250 of the sample) have been analyzed. Western immunoblots had been probed working with the mouse anti-p27Gag Ab KK64, which recognizes an epitope amongst aa 15180 and overlapping CE1 and…[Read more]

  • Kevin Copeland posted an update 4 weeks ago

    (B) HEK293T cells have been transfected with SIV pDNAs expressing p27CE1 (lane 1), p27CE2 (lane two), p57Gag (lane 3), Gag-Pro (lane four). Lane five contains a sample from mocktransfected cells. Proteins in the cell-associated (prime panel: 1/100 of your sample) and extracellular (bottom panel: 1/250 with the sample) have been analyzed. Western…[Read more]

  • Kevin Copeland posted an update 1 month ago

    Ermined. The DNA vaccine in macaques L986, R108 by means of R684 was administered employing the Elgen 1000 electroporation device, and in macaques T129 via T152 working with the CELLECTRA 5P device. No distinction in the level or nature on the induced CE-specific T cell responses was located making use of these electroporation devices, which…[Read more]

  • Proteins from the cell-associated (prime panel: 1/100 of the sample) and extracellular (bottom panel: 1/250 in the sample) have been analyzed. Western immunoblots had been probed making use of the mouse anti-p27Gag Ab KK64, which recognizes an epitope between aa 15180 and overlapping CE1 and CE2. Equal loading from the blots was controlled by…[Read more]

  • Kevin Copeland became a registered member 1 month, 2 weeks ago