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  • Sacha Greene posted an update 3 months, 1 week ago

    Teria. This pathway consists of a catechol branch (cat) and protocatechuate branch (pca). The pca genes in P. putida DOT-T1E are arranged in three operons [pcaRKFTBDC (T1E_0230 through T1E_0238), pcaGH (T1E_0829 and T1E_830), pcaJI (T1E_2058 and T1E_2059)], as can also be the case in other P. putida and P. syringae strains (Fig. S5). The cat genes encode the proteins responsible for catechol degradation and are organized in two clusters [catRBCA (T1E_5502 through T1E_5505) and catBCA (T1E_1744 by way of T1E_ 1746)] (Fig. S6), maintaining the gene order discovered in others P. putida strains and also in P. aeruginosa. The identity with the catBC plus a genes in both clusters is within the array of 79?two . Moreover, we should really mention that two other catA genes have been located, 1 of them with a higher degree of similarity for the KT2440 catA2 gene, which corresponded to ORF T1E_1057, that’s adjacent to the benRABCDK genes (T1E_1055 to T1E_1064) for benzoate degradation; even though the other catA allele corresponded to ORF T1E_5511. It should be noted that this allele is inside a cluster of genes which might be transcribed within the very same path and which encode genes for salycilate metabolism (T1E_5510 through T1E_5513). The genes involved in phenylacetate degradation had been also identified in P. putida DOT-T1E. You can find 16 genes encoding for phenylacetate degradation organized within a cluster (ORFs T1E_5587 to T1E_5603) and inside the cluster a series of prospective operons had been identified, i.e. the paaGHIJK genes (T1E_5590 by means of T1E_5594) that encode the ring-hydroxylating oxygenase enzyme, the paaABCDE genes that encode the b-oxidation enzymes, a potential phenylacetate transport method (paaLM) plus the regulatory program produced of paaXY, that correspond to T1E_5587 and T1E_5588 respectively. Homologous genes for degradation of homogentisate are also present in strain DOT-T1E. Homogentisate is catabolized by a central catabolic pathway that involvesFig. 4. Pathway for utilization of urea as an N source by P. putida. The genes that encoded the enzymes of these two pathways were identified according to BLAST analysis and comparison to proteins that carry out the indicated reactions.3 enzymes, homogentisate dioxygenase (T1E_1557), a newly identified putative maleylacetoacetate isomerase (T1E_1555) and fumarylacetoacetate hydrolase (T1E_1558). In this pathway homogentisate is funnelled to yield fumarate and acetoacetate. A look for hpa and gtd genes that encode genes belonging for the homoprotocatechuate and gentisate pathways yielded no benefits from the DOT-T1E genome, which suggests the absence of a meta ring-cleavage pathway for the degradation of homoprotocatechuate and gentisate. Pseudomonads strains are able to make use of a range of inorganic nitrogen sources. Within this regard 3 Nts and postdoctoral fellows. This has the added benefit of predicted transporters involved inside the uptake of ammonium had been identified. T1E incorporates ammonium into C skeletons using mainly the ATP-dependent activity of glutamine synthetase (GS) followed by the action of glutamate synthase (GOGAT). The genome of T1E encodes 4 GS (T1E_0118, 1260, 2050 and 4444) and four GOGAT enzymes (T1E_1644, 2053, 2506 and 3293). Strain T1E can use nitrate as an N source, that is lowered to ammonium using an assimilatory nitrate reductase (EC: 1.7.99.4) encoded by the T1E_4793 gene, that is within a cluster with nirB and nirD which encode an assimilatory nitrite reductase (EC1.7.1.4).